产品描述 概述Recombinant Mouse Pulmonary Surfactant-associated Protein D is produced by our Mammalian expression system and the target gene encoding Ala20-Phe374 is expressed with a 6His tag at the C-terminus. 使用说明This material is offered by Novin Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. 技术规格| Tag | C-6His | | 种属 | Mouse | | 表达系统 | Human Cells | | Accession# | P50404 | | Source | Human Cells | | Formulation_Description | Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, pH 7.4 . | | Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. | | Reconstitution | Dissolve the lyophilized protein in distilled water. | | Purity | Greater than 95% as determined by reducing SDS-PAGE. | | Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. | | Background | Pulmonary surfactant-associated protein D (SP D) is a 43 kDa member of the collectin family of innate immune modulators. Mouse SP D cDNA encodes a 19 aa signal sequence and a 355 aa mature region with a 25 aa N terminal linking region, a 177 aa hydroxyproline and hydroxylysine collagen like domain, a 46 aa coiled coil segment, and a 106 aa, C terminal collectin like C type lectin domain . SP D is found in serum, plasma, broncho alveolar lavage (BAL) fluid, and amniotic fluid. It also binds SIRP alpha and the calreticulin/CD91 complex on macrophages. SP D contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. It Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. It may participate in the extracellular reorganization or turnover of pulmonary surfactant. It binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. |
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