Human SHBG-试剂盒相关-ELISA 试剂盒；-北京诺梵生物科技有限公司

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Human SHBG

Human SHBG Quantikine ELISA Kit Summary

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Assay Length	4.5 hours
Sample Type & Volume Required Per Well	Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity	0.012 nmol/L
Assay Range	0.1 - 4 nmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity	Natural and recombinant human SHBG
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested
Interference	No significant interference observed with available related molecules.

Product Summary

The Quantikine^® Human SHBG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SHBG in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SHBG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SHBG showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SHBG.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SHBG

SHBG (Sex Hormone-binding Globulin), also known as ABP (Androgen-binding Protein), is a variably glycosylated, secreted, non-disulfide-linked homodimer. Male and female sex hormones bind SHBG with equal affinity. This binding protein can be found in the circulation where it regulates hormone access to target tissues. Human SHBG shares approximately 67% amino acid sequence identity with mouse and rat SHBG.

Long Name:	Sex Hormone-binding Globulin
Entrez Gene IDs:	6462 (Human)
Alternate Names:	ABP; ABPTestosterone-estrogen-binding globulin; androgen binding protein; MGC126834; MGC138391; SBP; sex hormone-binding globulin; Sex steroid-binding protein; SHBG; TEBG; Testis-specific androgen-binding protein; testosterone-binding beta-globulin; Testosterone-estradiol-binding globulin

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1、Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2、Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3、Add 50 μL of Assay Diluent to each well.

4、Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5、Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6、Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7、Aspirate and wash 5 times.

8、Add 100 μL Substrate Solution to each well.

9、Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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