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产品信息 >> 试剂盒相关 >> ELISA 试剂盒;
Human Decorin

Human Decorin DuoSet ELISA Summary

Assay   Type

Solid   Phase Sandwich ELISA

Format

96-well   strip plate

Assay   Length

4.5   hours

Sample   Type & Volume Required Per Well

100 μL

Sensitivity

0.03   ng/mL

Assay   Range

31.2 -   2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin   Plasma)

Specificity

Natural   and recombinant human Decorin

 

Cross-reactivity

<   0.5% cross-reactivity observed with available related molecules.< 50%   cross-species reactivity observed with species tested

Interference

No   significant interference observed with available related molecules.

Product Summary

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Decorin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Preparation and Storage

Shipping

The   product is shipped at ambient temperature. Upon receipt, store it immediately   at the temperature recommended below.

Storage

Store   the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Decorin

Decorin, also known as PG40 and PGS2, is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors basic FGF, GDF-8/Myostatin, TGF-beta, and WISP-1. It also binds and activates EGF R, ErbB4, and IGF-I R. Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression.

Long   Name:

Decorin

Entrez   Gene IDs:

1634   (Human); 13179 (Mouse)

Alternate   Names:

Bone   proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate   proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core   protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B

Assay Procedure

Refer to the product datasheet for the complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

1.     Prepare all reagents, standard dilutions, and samples as directed in the product insert.

2.     Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

3.     Add 50 μL of Assay Diluent to each well.

4.     Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

5.     Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

6.     Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

7.     Aspirate and wash 5 times.

8.     Add 100 μL Substrate Solution to each well.

9.     Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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