Human LOX-1/OLR1 DuoSet ELISA Summary Assay Type | Solid Phase Sandwich ELISA | Format | 96-well strip plate | Assay Length | 4.5 hours | Sample Type & Volume Required Per Well | 100 μL | Sensitivity | 4.17 pg/mL | Assay Range | 31.2 - 2,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine) | Specificity | Natural and recombinant human LOX-1/OLR-1 | Cross-reactivity | < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested | Interference | No significant interference observed with available related molecules. |
Product Summary This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human LOX-1/OLR-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet. Preparation and Storage Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Background: LOX-1/OLR1Lectin-like Oxidized Low-density Lipoprotein Receptor 1 (LOX-1), also known as Oxidized Low-density Lipoprotein Receptor 1 (OLR1), is a type II transmembrane receptor belonging to the class E scavenger receptor (SR-E) subfamily of the C-type lectin family. It binds and supports the internalization of multiple structurally unrelated macromolecules. It exists on the cell surface as covalent homodimers, which can further associate into non-covalently-linked oligomers. Cell surface LOX-1 can also be cleaved to release the soluble LOX-1 extracellular domain. Long Name: | Lectin-like Oxidized Low-density Lipoprotein Receptor 1 | Entrez Gene IDs: | 4973 (Human); 108078 (Mouse) | Alternate Names: | CLEC8A; CLEC8ASLOX1; C-type lectin domain family 8 member A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX1; LOX-1; LOX1ox LDL receptor 1; LOXIN; OLR1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; Ox-LDL receptor 1; SCARE1; scavenger receptor class E, member 1; SR-E1 |
Assay Procedure Refer to the product datasheet for the complete assay procedure. Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate. 1. Prepare all reagents, standard dilutions, and samples as directed in the product insert. 2. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal. 3. Add 50 μL of Assay Diluent to each well. 4. Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours. 5. Aspirate each well and wash, repeating the process 4 times for a total of 5 washes. 6. Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours. 7. Aspirate and wash 5 times. 8. Add 100 μL Substrate Solution to each well. 9. Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm. |
