Human C-Peptide Quantikine ELISA Kit Summary Assay Type | Solid Phase Sandwich ELISA | Format | 96-well strip plate | Assay Length | 2.5 hours | Sample Type & Volume Required Per Well | Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL) | Sensitivity | 2.88 pmol/L | Assay Range | 28.9 - 1,850 pmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine) | Specificity | Natural and synthetic human Insulin C-Peptide | Cross-reactivity | < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested | Interference | No significant interference observed with available related molecules. |
Product Summary The Quantikine Human Insulin C-Peptide Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human Insulin C-Peptide in cell culture supernates, serum, plasma, and urine. It contains synthetic human Insulin C-Peptide and has been shown to accurately quantitate the peptide. Results obtained using natural human Insulin C-Peptide showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Insulin C-Peptide. Preparation and Storage Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Background: C-PeptideInsulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes. Long Name: | Proinsulin C-Peptide | Entrez Gene IDs: | 3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine) | Alternate Names: | C-P; C-Peptide; IDDM2; ILPR; Insulin C-Peptide; insulin; IRDN; MODY10; Proinsulin C-Peptide; proinsulin |
Assay Procedure Refer to the product datasheet for the complete assay procedure. Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate. 1、Prepare all reagents, standard dilutions, and samples as directed in the product insert. 2、Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal. 3、Add 50 μL of Assay Diluent to each well. 4、Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours. 5、Aspirate each well and wash, repeating the process 4 times for a total of 5 washes. 6、Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours. 7、Aspirate and wash 5 times. 8、Add 100 μL Substrate Solution to each well. 9、Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm. |
