Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit Summary Assay Type | Solid Phase Sandwich ELISA | Format | 96-well strip plate | Assay Length | 4.5 hours | Sample Type & Volume Required Per Well | Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL) | Sensitivity | 2.18 pg/mL | Assay Range | 62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine) | Specificity | Natural and recombinant human Galectin-3BP/MAC-2BP | Cross-reactivity | < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested | Interference | No significant interference observed with available related molecules. |
Product Summary The Quantikine Human Galectin-3BP/MAC-2BP Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3BP in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human Galectin-3BP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human Galectin-3BP. Results obtained using natural human Galectin-3BP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3BP. Preparation and Storage Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Background: Galectin-3BP/MAC-2BPThe galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. Long Name: | Galectin-3 Binding Protein | Entrez Gene IDs: | 3959 (Human) | Alternate Names: | 90K; Basement membrane autoantigen p105; BTBD17B; galectin-3-binding protein; Galectin3BP; Galectin-3BP; L3 antigen; Lectin galactoside-binding soluble 3-binding protein; lectin, galactoside-binding, soluble, 3 binding protein; LGALS3BP; M2BP; Mac-2 BP; Mac-2-binding protein; MAC2BP; MAC-2BP; MAC-2-BP; serum protein 90K; Tumor-associated antigen 90K |
Assay Procedure Refer to the product datasheet for the complete assay procedure. Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate. 1、Prepare all reagents, standard dilutions, and samples as directed in the product insert. 2、Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal. 3、Add 50 μL of Assay Diluent to each well. 4、Add 50 μL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours. 5、Aspirate each well and wash, repeating the process 4 times for a total of 5 washes. 6、Add 100 μL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours. 7、Aspirate and wash 5 times. 8、Add 100 μL Substrate Solution to each well. 9、Add 100 μL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm. |
